Details, Fiction and high performance liquid chromatography
Details, Fiction and high performance liquid chromatography
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ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
The sample separation happens from the column for which temperature should be constant. So to take care of the consistent temperature, a column is put within the column oven. The interaction of the person components and the stationary section start to occur. In case the stationary period as well as individuals have the same nature, i.e., both are polar, then the polar compound will connect with it for some time.
In this segment we consider the fundamental plumbing necessary to transfer the cellular stage with the column and also to inject the sample into the mobile period.
As being a typical rule, a two unit transform while in the polarity index corresponds to an roughly 10-fold adjust inside a solute’s retention issue. Listed here is a simple example. If a solute’s retention issue, k
シリカゲルの粒子径が小さければ小さいほどピークの分離性は良くなるが、送液に必要なポンプの圧力が高くなる。そのため、ポンプ－インジェクター間、インジェクター－カラム間の配管の耐圧を上げたり、カラム自体を比較的高温の下にさらして溶媒の粘度を下げ、抵抗を小さくする工夫をしている。
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The working tension inside an HPLC is adequately high that we are not able to inject the sample in the cell phase by inserting a syringe by way of a septum, as is feasible in gas chromatography. Rather, we inject the sample using a loop injector
The obvious way to respect the theoretical and the sensible specifics discussed During this section would be to read more carefully examine a typical analytical method.
Ion-exchange chromatography relies about the separation of substances based mostly on their charge. The stationary period consists of billed teams that attract and keep oppositely charged ions from the sample.
- 분석물의 분리여부는 고정상(컬럼)과 이동상의 조합에 의해 결정합니다.(실제 시료 측정에서는 시료 중에 분석물 이외의 오염물질에 존재하는 경우가 많아 분석자는 그 시료의 측정에 최적인 분석 조건의 검토가 필요합니다.
Two troubles often shorten the life time of HPLC working the analytical column. 1st, solutes that bind irreversibly on the stationary section degrade the column’s performance by reducing the amount of stationary phase accessible for effecting a separation. 2nd, particulate content injected with the sample may well clog the analytical column.
Sample carryover: Sample components can continue to be while in the system just after an injection, causing them to look in subsequent injections as ghost peaks. Guarantee right rinsing of your injection system involving injections. Consider increasing the wash quantity or using a stronger clean solvent.
The injector introduces a precise volume in the sample Remedy in to the mobile section stream. Quite a few injection procedures exist, with loop injection currently being a common method.